dual colour elispot kit  (Cellular Technology Ltd)

Journal: Vaccines

Article Title: An Advax-Adjuvanted Inactivated Cell-Culture Derived Japanese Encephalitis Vaccine Induces Broadly Neutralising Anti-Flavivirus Antibodies, Robust Cellular Immunity and Provides Single Dose Protection

doi: 10.3390/vaccines9111235

Figure Lengend Snippet: Addition of Advax enhances Th1-associated cytokine production to ccJE vaccine. C57BL/6 mice ( n = 10/group) were immunised and boosted after 3 weeks with ccJE (50 ng) or mbJE (50 ng) alone or formulated with Advax (1 mg) or alum (30 µg). Spleens were collected 3 week post last immunisation and pooled for each group. Splenocytes were stimulated with ccJE vaccine (1 μg/10 6 cells) or flaviviruses (JEV, WNV, DENV1 or DENV2) at a MOI of 0.1 for 10 6 cells for 4 days. Cytokines, ( A ) IL-5, ( B ) IFN-α, ( C ) IL-3, ( D ) IL-17, ( E ) IL-4 and ( F ) IFN-γ were measured in culture supernatant using a mouse multiplex immunoassay and a mouse interferon-α ELISA kit.

Article Snippet: Antigen-specific IFN-γ and IL-17A ELISPOT assays were conducted with Mouse IFN-γ/IL-17 Dual-Colour ELISPOT Kit (R&D Systems) and Mouse IL-5 ELISpot Kit (R&D Systems) respectively, according to the manufacturer’s instructions.

Techniques: Multiplex Assay, Enzyme-linked Immunosorbent Assay